HEMOSTASIS ACHIEVEMENT REFLECTING THE INFLAMMATORY STATUS OF THE PULP BASED ON CYTOKINE LEVELS: A COHORT STUDY

Aim:To investigate whether hemostasis at the exposure site reflect the level of inflammation at canal orifices of primary teeth with carious pulp exposures through measuring the cytokines levels at the exposure sites and canal orifices.Materials and methods:This study was conducted on (30) children aged 5-7 years with deep carious lesions in their primary molars.It included 41 carious primary molars at which hemostasis at the exposure site was achieved within five minutes (group I), and 41 carious primary molars at which hemostasis at the exposure site was not achieved within five minutes but was achieved at the canal orifice within five minutes (group II).To assess inflammatory markers at the exposure site and canal orifice, a pulpal blood samples were analyzed for IL-2, IL-6 and IL-8 levels (pg/ml) using ELISA.Data were statistically analyzed. Results:The levels of IL-2, IL-6 and IL-8 at the exposure site in group (I) revealed significantly difference between all interleukins (P<0.0001).The levels of IL-8 in group (I) at the canal orifice, group (II) at the exposure site and at the canal orifice showed significantly the lowest while it revealed insignificant difference between IL-2 and IL-6.Conclusions:IL-2, IL-6 and IL-8 cytokine expression in primary molars increased in exposure sites and increases with caries progression at canal orifices.