Toxicological Assessment of Okra Varieties (Clemson spineless, Ex-Samaru, and NHAE-74) in Wistar Rats

ABSTRACT Since plants make up the majority of the food consumed by living things, their metabolisms generate a variety of poisons that can cause illness and occasionally even death. In order to determine the safety of three improved okra seed variants, this journal assesses their toxicity. Twenty-eight albino rats weighing between 132 and 136 g were split into four groups at random for the investigation. For twenty-eight days, group 1 (control) was fed their regular diet of chicken feed, while groups 2, 3, and 4 were fed a mixture of a 1:3 ratio of chicken feed and (75% okra seed), a variety of okra seed (Clemson spineless, Ex-samaru, and NHAE-74, respectively). The mass difference of each rat was measured at the end of each seven days. After twenty-eight days, the rats were killed and their blood was extracted by cardiac puncture. The kidney function parameters, creatinine, urea, Na+, K+, Cl-, and HCO3- are within their normal range. The liver function parameter of Total protein (3–6.5), albumin (2.87–2.93), and globulin (2.23-2.85) concentrations (g/dL) are all within the normal range. The levels of direct bilirubin (0.14-0.17), total bilirubin (0.39-0.43), and AST, ALT, and ALP (mg/dL) are all within the normal range. In hematological studies, every parameter that was examined fell within the normal range. The seeds are safe to eat because feeding on them causes an increase in body weight without causing anemia, kidney, or liver problems. Keywords: Toxicity, Liver Function Test, Kidney Function Test, Bilirubin, and Aspartate Aminotransferase.