Evaluation of the role of melatonin for protect testicular tissues from harmful effects of diclofenac in rat male

The aim of the current study is to evaluate the effect of melatonin as an protect testicular tissues after the harmful effects of diclofenac. Thirteen rats were used in this study, which lasted for month. Divided into 6 groups, each group contain 5 rats. The control group G1 (vehicle treated) received normal saline (0.1 ml/day). The Second group G2 (5 rats) treated with Diclofenac. (0.7 mg/Kg/B.W), The Third group G3 (5 rats) was treated with Melatonin. (0.07 mg/Kg/B.W), The Fourth group G4 (5rats) was treated with Diclofenac.(1.4 mg/Kg/B.W),The fifth group G5(5 rats) was treated with Melatonin + Diclofenac.(0.7 mg/Kg/B.W +0.07 mg/Kg/B.W),and The sixth group G6 (5 rats) was treated with Melatonin + Diclofenac. (1.4 mg/Kg/B.W +0.07 mg/Kg/B.W). The results indicate that diclofenac sodium induces significant reproductive dysfunction in male rats, characterized by degeneration of spermatogenic cells and Leydig cells. Melatonin administration appears to mitigate some of these adverse effects, particularly at the lower dose of diclofenac (0.7 mg/Kg/B.W), suggesting its potential as a protective agent against diclofenac-induced reproductive toxicity. Further studies are warranted to explore the mechanisms underlying these protective effects and to optimize treatment protocols. In conclusion, the reproductive toxicity effects of DF these effects on testicular tissues could be attenuated by treatment with melatonin.