Isolation and Characterization of Novel Phenolic Compounds from theAerial Parts of Anaphalis triplinervis (Sims) C.B. Clarke

Medicinal plants are the best source of bioactive molecules and could serve as an appreciable source of many medicines.Anaphalis triplinervis (A. triplinervis), belonging to the family Asteraceae, is a perennial shrub that commonly grows inshady, moist habitats at elevations of around 2200 meters, and is generally found in India, Pakistan, and several other partsof the world. A. triplinervis, generally known as Bugla in Hindi and triple-veined pearly everlasting in English. The aerialparts of the plant are utilized to aid many diseases as well as serve as an important ethnomedicine. A. triplinervis isfrequently used in bug bites, snake bites, as an anti-inflammatory, anti-asthmatic, anti-coughing, expectorant, sedative, andin convulsions. Isolation of ethyl acetate extract from A. triplinervis was chosen for the current investigation based on thin-layer chromatography and the first phytochemical screening. The ethyl acetate extract of the aerial parts of A. triplinerviswas subjected to column chromatography following successive solvent extractions to isolate individual compounds. Everyfraction of 20ml has been collected, and a total of 491 fractions were taken. Two phenolic compounds were isolated andinitially confirmed by spraying a dilute ferric chloride solution on TLC plate. Further structure elucidation has beenconducted by utilizing several spectrophotometric methods such as UV, IR, 1H &13C NMR, along with mass spectroscopy(MS). Based on spectral analysis, the compound was identified as Quercetin 3,7-di-O-rhamnoside, a flavonoid, along withcaffeic acid, a phenolic acid.