CRISPR-Induced Autovaccination for Ewing Sarcoma: Combined Genome Editing and Dendritic Cell Vaccine Strategy

This work proposes a novel therapeutic strategy termed "CRISPR-induced autovaccination" for Ewing sarcoma. The approach combines in vivo CRISPR/Cas9 genome editing targeting the EWSR1-FLII fusion oncogene with photodynamic therapy (PDT), coupled with an ex vivo personalized dendritic cell vaccine. The dual-arm strategy aims to achieve immediate tumor cytoreduction while establishing durable systemic immunity through autovaccination.