EVALUATION OF HEMOCOMPATIBILITY AND CYTOCOMPATIBILITY NOVEL CISSUS QUADRANGULARIS, BIOCERAMICS AND TENDON EXTRACELLULAR MATRIX INCORPORATED SCAFFOLDS FOR PERIODONTAL BONE REGENERATION

This study presents the fabrication and evaluation of a novel bioactive scaffold integrating Cissus quadrangularis extract, carrageenan, tendon-derived extracellular matrix (ECM), and bioactive ceramics such as silver hydroxyapatite and silver tricalcium phosphate for periodontal bone regeneration. The scaffold’s biocompatibility was evaluated using MTT assay, while mineralization capacity was assessed via Alizarin Red S staining. Hemocompatibility was tested to ensure safe interaction with blood components. Furthermore, osteogenic potential was investigated through quantitative PCR analysis of key markers: RUNX2, ALP, OCN, COL1A1, and BMP2 using osteoblastoma cell lines. Results demonstrated high cell viability, minimal haemolytic activity, enhanced mineral deposition, and significant upregulation of osteogenic genes, particularly in group 4 containing tendon ECM and silver tricalcium phosphate. The findings support the scaffold’s potential for clinical use in periodontal bone defect repair, offering a cost- effective and multifunctional approach for regenerative dentistry.