Mangosteen Peel Extract and Mahogany Seed Extract Combined: Antihyperglycemic Activity and Acute Toxicity

Diabetes mellitus (DM) is characterized by hyperglycemia. Herbal medicines, such as mangosteen rind extract (MRE) and mahogany seed extract (MSE), have antihyperglycemic potential. This study aims to evaluate the antihyperglycemic effect of the MRE+MSE combination, which has been standardised, and determine its acute toxicity. Mice that had been induced by alloxan were divided into 6 groups to observe the antihyperglycemic effect: a negative control group, a positive control, and 4 groups MRE+MSE. The mice had their blood glucose levels checked and measured again on days 6, 9, 12, and 15. Meanwhile, the acute toxicity test used doses of 5, 30, 500, 2000, and 5000mg/kg BW. Animal behavior was monitored for toxic symptoms, alongside daily body weight changes over 14 days. On the final day, mice were sacrificed, dissected, and their organs (heart, liver, stomach, intestines, lungs, spleen, and kidneys) were collected for analysis.Data analysis using one-way ANOVA and Tukey's post hoc test (sig. p<0.05). A statistical analysis showed that MRE+MSE comply with quality standards and had an antihyperglycemic effect starting on the sixth day of observation at dose 3 (MRE 280mg/kg BW and MSE 125mg/kg BW). It was almost identical to the effect of the positive control group. The combination of other dosed extracts also demonstrated this effect on the 9th to 15th days of observation. This combination of extracts did not show toxic effects up to a dose of 5000mg/kg bw for 14 days of observation (LD50 > 5000mg/kg bw, or non-toxic). It can be concluded that the combination of MRE and MSE is supported by consistent quality standards, and its antihyperglycemic effect can treat DM effectively. It is also in the non-toxic category (safe to use for a certain period of time).