Antibiofilm Activity of Active Isolates of Telang Ternate (Clitoria ternatea L.) against Oral Pathogenic Bacteria

Biofilms are a serious health problem that causes various chronic infections. Oral biofilm infections such as dental caries, periodontitis, halitosis, and gingivitis are caused by oral pathogenic bacteria such as Streptococcus mutans, Lactobacillus acidophilus, Pseudomonas aeruginosa, and Staphylococcus aureus. This study aims to evaluate the antibiofilm activity of active isolates of Telang Ternate (Clitoria ternatea) plant against oral pathogenic bacteria. The methods used include microdilution test to measure the ability of active isolates to inhibit mid-phase (24hours) and maturation (48hours) biofilm formation, as well as biofilm eradication test. The results showed that at a concentration of 1% b/v, the active isolate of Telang Ternate was able to inhibit biofilm formation of S. mutans by 85.17%, L. acidophilus by 83.04%, P. aeruginosa by 80.67%, and S. aureus by 78.62% after 24hours. Biofilm inhibition in the maturation phase also showed significant effectiveness, although slightly lower compared to the mid-phase. The conclusion of this study suggests that the active isolate of Telang Ternate has potential as a natural antibiofilm agent that is effective in inhibiting the formation and eradication of biofilms by oral pathogenic bacteria. Further research is needed to identify the active components in Telang Ternate responsible for this antibiofilm activity and its mechanism of action.