UV-Vis Spectrophotometric Method of Amantadine Hydrochloride Analysis: Method Development, Validation and Application to Solubility Studies

The goal of the current work was to develop a sensitive UV-Vis spectrophotometric method for estimation of amantadine hydrochloride. A safe, precise, quick, easy, affordable and effective approach by derivatizing an aliphatic cyclic primary amine drug moiety was designed, since it lacks a chromophoric functional group. The 1,2-naphthoquinone-4-sulphonate reagent was used for derivatization due to its high affinity towards primary amine group. The maximum absorption wavelength obtained was 433nm. The limit of detection obtained was 0.35μg/ml. The linearity was assessed in between 2-20μg/ml with good correlation coefficient of 0.9983. Each parameter was analyzed within the developed range, and the percent relative standard deviation was found to be below 2%, which indicated accuracy of the developed method for different parameters. The validated approach was applied for the analysis of amantadine hydrochloride's solubility in solid lipids (glyceryl monostearate, stearic acid and beeswax), liquid lipids (eucalyptus oil, oleic acid, isopropyl myristate and castor oil) and surfactants (Tween-80, Tween-20 and Span-80). The drug showed highest solubility in Glyceryl monostearate (40.36mg/g), eucalyptus oil (3.45mg/ml) and Tween-20(3.47mg/ml). The resulted outcomes demonstrated the method's applicability for quantitative estimation of amantadine hydrochloride in future studies. Solid lipids, liquid lipids and surfactants may be analyzed precisely by the developed method.