Silvar Nanoparticles Synthesis from Endophytic Curvularia Colbranii from Medicinal Leaf Sample of Acalypha Indicain Srivilliputhur, Tamil Nadu, India

Silver nanoparticle synthesis employing endophytic Curvularia colbranii from a leaf sample of Acalypha indica. The color shift from pale yellow to dark brown was used to identify and observe silver nanoparticles. The development of turbity signifies the reduction of silver ions (Ag+) to silver atoms (Ag0), which is followed by the formation of silver nanoparticles (AgNPs). Following the color changes, the solution containing the Curvularia colbranii silver nanoparticles was exposed to UV-visible absorption spectroscopy 24 hours later. The separation of the nanoparticle extract from the pure Curvularia colbranii extract was verified by the appearance of two distinct clean peaks for the Ch-AgNPs nanocomposites at 500 nm and 262 nm. The FTIR Spectra analysis was carried out for the silver nanoparticle sample and has the absorption peak. Morphological characterization at the nanoscale to micrometer scale is accomplished using scanning electron microscopy (SEM). The effective synthesis of silver nanoparticles is ensured by the presence of silver being verified by the silver-specific peaks seen in the EDX spectrum, antibacterial activity of the nanoparticle extract and obtained the zones of inhibition (ZOI) against Escherichia coli, Staphylococcus aureus. The produced silver nanoparticle's antifungal properties were tested against Aspergillus flavus and Aspergillus niger.