Long-Term Effects of Contramal on Reproductive Physiology of Female Albino Mice

Abstract: The study aims to explain the dose-dependent possible deleterious effects of administration of Contramal, the known analgesic on the reproductive physiology of female albino mice. Contramal at a dose of 0.05mg/kg administered intramuscularly daily once for 60 days. Alterations in hormonal parameters like estrogen, progesterone, follicular stimulating hormone and Luteinizing hormone were investigated. Decrease in body and tissue weight were also recorded. The present study investigates the dose-dependent deleterious effects of Contramal (Tramadol), a commonly prescribed analgesic, on the reproductive physiology of female albino mice. Experimental animals were intramuscularly administered Contramal at 0.05 mg/kg once daily for 60 days, while controls received saline. The study assessed alterations in body and organ weights along with hormonal parameters including estrogen, progesterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH). Results indicated significant reductions in body weight, ovary and uterus weights, as well as considerable alterations in the hormonal profile of treated mice. Long-term administration of Contramal markedly decreased steroidogenic activity, suggesting potential anti-steroidal effects. The findings highlight that chronic exposure to Contramal can adversely affect female reproductive health by disrupting the hypothalamic-pituitary-gonadal axis and altering biochemical as well as morphological parameters