High-Throughput UPLC Method for Accurate Quantification of Betahistine in Pharmaceutical Tablets

A simple, novel, sensitive and rapid ultra-performance liquid chromatographic (RP-UPLC) method has been developed and validated for quantitative determination of Betahistine in bulk and tablet formulations. The chromatographic development was carried out on Water C18 (4.6mm X 250 mm; 5 microns) column, with mobile phase consisting of Buffer : ACN 600ml:400 ml v\v with pH adjust to 3.5.. The flow rate was 1 ml/min and the effluents were monitored at 254 nm. The retention time was found to be 1.951 min. The method was validated as per International Conference on Harmonization Guideline with respect to linearity, accuracy, precision, and robustness. The calibration curve was found to be linear over a range of 31–95 μg/mL with a regression coefficient of 0.9999. The method has proved to be of high sensitivity and specificity. The results of the study showed that the proposed RP-UPLC method was simple, rapid, precise and accurate which is useful for the routine determination of Betahistine in bulk drug and in its pharmaceutical dosage form.