Genotyping of Gleditsia triacanthos L. based on RbcL gene expression and using ISSR markers in arid climate conditions

Background.Fighting against desertification is one of the priorities in the world.Gleditsia triacanthosL. is a promising species for afforestation as it has high drought resistance. Genotyping ofG. triacanthoshas not been previously conducted based on the study of photosynthetic productivity by evaluating the expression of the large subunit gene of RuBisCo -RbcLand linking it to ISSR markers. Purpose.To conduct the genotyping of individuals in theG. triacanthospopulation by evaluation the quantitative expression of the large subunit gene of RuBisCo -RbcLand their linkage to ISSR markers. Materials and methods.The study was carried out at the arboretum of the FSC of agroecology RAS, where 10 individuals ofG. triacanthosin good vital state were selected. The expression ofRbcLwas evaluated using RT-PCR. ISSR analysis was performed to examine the genetic structure of the studiedG. triacanthosindividuals. Statistical analysis of experimental data was performed using the software package Statistica 12.0 (StatSoft, USA) and POPGENE version 1.31. Results.Based on the results of theRbcLexpression evaluation, individuals with different transcriptional activity were identified: 20% of G. triacanthosindividuals had high expression, 40% had moderate expression, and 40% had low expression. Effective primers from the UBC group, 836 and 873, were identified during ISSR analysis inG. triacanthosindividuals. Genotyping by ISSR revealed two groups ofG. triacanthosindividuals with high and lowRbcLexpression. Conclusion.The acquired data can potentially be used in molecular breeding ofG. Triacanthosto obtain not only drought-resistant genotypes, but also genotypes with high photosynthetic productivity to address agroforestry tasks.