The Dublin lens: a Cc = 1.0 mm objective lens intended for CryoEM at 100 keV

Abstract We have designed, fabricated and tested a lens with a chromatic aberration coefficient (Cc) of 1.0 mm, a 4.0 mm pole-gap and 2.0 mm bore that is wide enough to accommodate an anti-contamination system and an objective aperture. This lens extends the temporal-coherence envelope of the electron microscope beyond 2 Å, using a low-cost Schottky FEG. We hope that this lens design can be used to improve all 100 keV electron microscopes designed for single-particle electron cryomicroscopy (cryoEM).

Institutions

Publication Details

Journal
Methods in microscopy
Published
2025-09-23
DOI
https://doi.org/10.1515/mim-2025-0019
Citations
1
Primary Topic
Agriculture, Land Use, Rural Development
Type
article
Field-Weighted Citation Impact
4.83
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article

The Dublin lens: a Cc = 1.0 mm objective lens intended for CryoEM at 100 keV

1 citations
Methods in microscopy
Agriculture, Land Use, Rural Development
4.83
article

The Dublin lens: a Cc = 1.0 mm objective lens intended for CryoEM at 100 keV

article en
1 citations

Abstract

Abstract We have designed, fabricated and tested a lens with a chromatic aberration coefficient (Cc) of 1.0 mm, a 4.0 mm pole-gap and 2.0 mm bore that is wide enough to accommodate an anti-contamination system and an objective aperture. This lens extends the temporal-coherence envelope of the electron microscope beyond 2 Å, using a low-cost Schottky FEG. We hope that this lens design can be used to improve all 100 keV electron microscopes designed for single-particle electron cryomicroscopy (cryoEM).

Methods in microscopy
MRC Laboratory of Molecular Biology (GB), Trinity College Dublin (IE), Turbo Power Systems (United Kingdom) (GB), Advanced Materials and BioEngineering Research (IE), Tris Pharma (United States) (US), University of York (GB)
Openalex Percentile: Top 4%
Agriculture, Land Use, Rural Development
4.83
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