THE CONTENT OF BIOLOGICALLY ACTIVE AND MINERAL SUBSTANCES IN THE COLLECTION OF ANGIOPROTECTIVE ACTION

Angioprotective collection includes raw materials: Aesculi hippocastani semina, Calendulae officinalis flores, Aroniae melanocarpae sicco fructus, Bupleuri multilervis herba, Filipendulae ulmariae flores, Fragariae vescae folia. In terms of the content of biologically active substances (BAS), the raw materials of the collection components met the requirements of regulatory documents. The purpose of the study was to determine the quantitative content in the collection of BAS, which are the main contribution to the development of angioprotective action. The task of the study was to determine the content of BAS of a phenolic nature, amino acids, ascorbic acid, carotenoids, saponins, elements. The quantitative content of flavonoids was determined by spectrophotometric method in the differential version, the analytical wavelength 415 nm was determined, the percentage content of flavonoids in terms of rutin was calculated. The total flavonoid content was 3.28–4.94%. The content of phenylpropanoids (3.32–3.67%), tannins (4.36–5.12%), anthocyanins (0.54–0.80%), triterpene saponins (0.95–1.08%), amino acids (6.24–6.42%), carotenoids (0.08–0.09%) was determined spectrophotometrically; by titrimetric methods – ascorbic acid (0.30–0.38%), organic acids (1.75–1.90%). 8 macronutrients (calcium 10800 mg/kg, potassium 24000 mg/kg, magnesium 3600 mg/kg), 20 micronutrients (manganese 53 mg/kg, zinc 2 mg/kg, selenium 0.13 mg/kg) were identified. The standards for the content of flavonoids in the collection are not less than 3.0%, the sum of phenylpropanoids is not less than 2.5%, total ash is not more than 11%, ash insoluble in hydrochloric acid is not more than 3.5%. The established parameters determine the quality of collection of angioprotective action.