Molecular Diagnosis and Genotype Distribution of Human Papillomavirus among Women in Nepal

Abstract: Setting: Intrepid Nepal Pvt Ltd, Thapathali, Kathmandu, Nepal between August 2020 and August 2023 Objectives: To determine the prevalence of Human papillomavirus and their type specific distribution among women Design: laboratory based, cross sectional study Results: Among the 6474 samples received, 574 were identified as Human papillomavirus. Overall, the prevalence of the HPV isolates were found to be 9.4%. Genotyping of 51 women sample were done. All infection with High-risk type. 66.6% single infection and 33.3% multiple infection. Most common HR HPV were HPV 16(33.3%), followed by HPV 56(17.6%), 66(15.6), 18(13.7%), 58(13.7%), 59(11.7%), 31(7.8%), 39(7.8%), 51(5.8), 52(5.8%), 68(5.8%). Higher HPV prevalence among young adult (20-39 years), followed by middle age adult and senior adult. Conclusion: Our results highlight the current status of HPV prevalence and genotypes among women in Nepal. The highest prevalence was found among 20-30 years. HPV vaccine is strongly recommended for regular immunization before the onset of sexual activities 9-13 years to reduce the burden of cervical cancer.