A Review on Moving Boundary Electrophoresis

Arne Tiselius, made a special machine called Tiselius apparatus to separate chemicals using electric current. Electrophoresis is defined as the migration of the charged particles through solution under the influence of an external electrical field. It has a wide application in separating and in analyzing the biomolecules such as protein, plasmids, DNA and RNA. Electrophoresis in 1930s types include capillary electrophoresis, immunoelectrophoresis, isoelectric focusing and isotachophoresis. Moving boundary electrophoresis is the method that allows the charged species to migrate within a free moving solution in the absence of supporting medium. Capillary electrophoresis works by separating molecules based on how fast they move in an electric field. Immunoelectrophoresis is a technique in which an antigen mixture is separated into its component parts. Isotachophoresis technique is used for the separation of ionic species based on their effective mobilities. In isoelectric focusing the charged molecules are separated based on their isoelectric point.