Analytical Methods for Determining the Peroxide Value of Hemp Seed (Cannabis Sativa L.) Oil

The peroxide Value is the number that expresses, in milli equivalents of active oxygen, the quantity of peroxide contained in 1000 g of the substance. Hemp seed oil containing essential omega fatty acids and proteins. This organic hemp seed oil has a pleasant nutty smell, deep green hue, and absorbs well into the skin. Hemp-seed oil has several positive effects on skin problems such as dryness and those related to the aging process. Hemp seed oil sample were peroxide value (POV) assayed using a standard starch solution, American Oil Chemist’s Society (AOCS) Method Cd 8-53, and used as a verified reference method for peroxide determination. Peroxide values (POV) is a standard test in the food and cosmetic industry to ensure product quality and self-life. In this study, peroxide value in Hemp Seed (Cannabis Sativa L.) oil has been determined by iodometric titration method using acetic acid-chloroform reagent. The average result is less than 4.0 meq O2 / Kg. Relative repeatability Standard Deviation %RSD is 2.64 %. The average result meets the specification.