To study plant Calotropis gigantea extract for anti-inflammatory activity

The leaves of Calotropis gigantea have a long history of use in treating various conditions, including edema, cancer, wounds, recurrent fevers, paralysis, and joint pain. Additionally, they serve as an antispasmodic and anti-asthmatic, and are applied externally for ailments such as leprosy, eczema, boils, ulcers, scabies, and hemorrhoids. While there are indications of positive anti-inflammatory effects, research has yet to conclusively demonstrate these properties. This study aims to assess the anti-inflammatory potential of an extract from Calotropis gigantea leaves using an in vitro method. The extraction process involved sequentially using petroleum ether (60–80°C), chloroform, ethyl acetate, n-butanol, ethanol, and distilled water. The resulting extract was then evaluated for its anti-inflammatory efficacy through a slightly modified albumin denaturation inhibition assay. For comparison, etoricoxib was used as the standard reference drug at a dosage of 100 mg/kg. The ethanolic extract of Calotropis gigantea leaves exhibited a denaturation Inhibition percentage of 85.71%, comparable to that of etoricoxib, suggesting that the extract has significant anti-inflammatory properties.