Impact of Cinnamon Silver Nanoparticles on Cognitive Function, Pain Sensitivity, and Biochemical Markers in Rats

The study investigated rat behavior for 7 and 14 days, observing various tests. In the Morris water maze test, experimental rats exhibited hyperactivity and impaired spatial learning and memory compared to controls, with significant differences observed. Similarly, in the Rota rod test, experimental rats displayed enhanced neuromuscular coordination over both time periods. Conversely, control rats showed greater resistance to thermal pain than experimental rats in the Tail flick test. In the Pain test, experimental rats initially exhibited more nociception than controls at 7 days, but this trend reversed at 14 days. Finally, in the Hot plate test, experimental rats displayed increased nociception to heat compared to controls at 14 days but not at 7 days. These findings suggest distinct alterations in behavior and pain sensitivity in rats over varying exposure durations. The study also examined the biochemical alterations in exposed rats compared to controls. Acetylcholine esterase activity decreased in the brain, liver, and kidney of experimental rats. Meanwhile, LDH activity significantly increased in these tissues over the 14-day period. Conversely, SDH activity decreased significantly in all experimental tissues. Protein estimation revealed a notable increase in protein levels in the brain, liver, and kidney of experimental rats compared to controls. These findings suggest significant biochemical alterations in enzyme activity and protein levels in response to experimental conditions, indicating potential shifts in metabolic processes and cellular function.