Evaluation of Phytochemical, Physiochemical, And Microbial Safety of The Siddha Formulation Venpoosani Legiyam: A Scientific Validation of Its Traditional Application

Background: Traditional Siddha Medicine Venpoosani Legiyam is used for its supposed health benefits, especially in dealing with oxidative stress, inflammation, and other connected conditions. Through in-depth biochemical, phytochemical, physiochemical, and microbiological tests, this study aims to give medicine a scientific base for its possible use. Methodology: Alkaloids, flavonoids, and glycosides were found in phytochemical screening, whereas biochemical analysis tested for important components and chemicals. Stability was determined by physiochemical study of moisture, total ash, and extractive values. Microbial load and aflatoxin testing followed WHO requirements for safety, testing for Escherichia coli, Salmonella spp., and Staphylococcus aureus. Results: Biochemical and phytochemical analyses showed high quantities of antioxidant and anti-inflammatory flavonoids and phenols. Physiochemical tests showed a stable composition with appropriate moisture and ash levels. Microbial investigation showed no infections or aflatoxins, and bacterial and fungal levels met WHO guidelines. Conclusion: Venpoosani Legiyam appears to be safe and stable, having bioactive components supporting its traditional medicinal use. Lack of pollutants and quality requirements proves its suitability for ingestion. This study supports Venpoosani Legiyam's complementary therapeutic potential, with future clinical trials required to assess its efficacy in modern healthcare.