Assessment of Hibiscus rosa-sinensis extract for Haematological recovery in Phenylhydrazine induced anaemia in Mus musculus

Anaemia is a prevalent global health concern, particularly among pregnant women and children, with iron deficiency being the leading cause. Hibiscus rosa-sinensis (China-rose) has demonstrated potential in mitigating anaemia due to its hematopoietic and iron-enhancing properties. This study investigates the effects of Hibiscus rosa-sinensis bark extract on phenylhydrazine-induced anaemia in Swiss albino mice( Mus musculus). Acute and sub-acute toxicity studies established the safety of the extract, determining non-toxic doses of 400 mg/kg and 800 mg/kg body weight. Experimental groups included normal controls, anaemia-induced controls, and treatment groups receiving Hibiscus rosa-sinensis extract of different doses along with synthetic drug administration of ferrous sulphate for comparison. Haematological parameters such as haemoglobin (Hb), hematocrit (Hct), red blood cell (RBC) count, white blood cell (WBC) count, and platelet count were assessed. Phenylhydrazine administration significantly reduced Hb levels, Hct, and RBC count, while increasing WBC count and neutrophil percentage, confirming the induction of haemolytic anaemia. Treatment with Hibiscus rosa-sinensis extract significantly improved haematological parameters, enhancing erythropoiesis and restoring RBC indices. The findings indicate that Hibiscus rosa-sinensis extract has promising therapeutic potential in anaemia management, warranting further clinical evaluation for human applications.