ORAL ACUTE TOXICITY STUDIES OF THE AQUEOUS FRUIT EXTRACT OF ANNONA MURICATA IN ALBINO RATS

Abstract: The acute toxicity of aqueous fruit extract of Annona muricata was evaluated in Swiss albino rats. The rats were randomly distributed into four groups of three animals each for the first phase. The groups were respectively administered orally aqueous fruit extract of Annona muricata at 0, 10, 100, and 1000 mg/kg body weight in a single dose. In the second phase of the experiment, the albino rats were divided into four groups of 3 animals each and were orally administered single doses of the extract at 0, 1600, 2900, and 5000mg/kg body weight and monitored frequently for 24 hours and 14 days. No death was recorded in each group. After the oral intubation, no mortality was observed in the albino rats. All the animals showed a positive gain in body weight throughout the study. The oral LD50 of aqueous fruit extract of Annona muricata was estimated to be equal to or below 5000mg/kg body weight, and the plant extract was concluded to be safe and non-toxic when taken through the oral route. The LD50 value, defined as the statistically derived dose that, when administered in an acute toxicity test, is expected to cause death in 50% of the treated animals in a given period, is currently the basis for toxicologic classification of chemicals. Keywords: Oral Acute Toxicity, Annona muricata, Aqueous Fruit Extract, Albino Rats. Title: ORAL ACUTE TOXICITY STUDIES OF THE AQUEOUS FRUIT EXTRACT OF ANNONA MURICATA IN ALBINO RATS Author: AMARACHI M. ACHEBE, ALOYSIUS C. ENE, BLEESING A. MONDAY, FORGIVE C. EGEGBULA, MAUREEN O. ARINZE International Journal of Recent Research in Interdisciplinary Sciences (IJRRIS) ISSN 2350-1049 Vol. 12, Issue 3, July 2025 - September 2025 Page No: 1-10 Paper Publications Website: www.paperpublications.org Published Date: 08-September-2025 DOI: https://doi.org/10.5281/zenodo.17077447 Paper Download Link (Source) https://www.paperpublications.org/upload/book/ORAL%20ACUTE%20TOXICITY%20STUDIES-08092025-4.pdf