AMELIORATION OF ETHYLENE GLYCOL-INDUCED HEPATOTOXICITY BY AVOCADO PEAR SEED EXTRACT IN A RAT MODEL

This study investigated the potential hepatoprotective effects of ethanolic extracts of avocado pear seed (ASE) against ethylene glycol (EG)-induced liver toxicity in male Wistar rats. Twenty-five rats were divided into five groups: a normal control, a positive control exposed to EG (0.1 ml/kg body weight) for seven days, two treatment groups receiving ASE at doses of 100 mg/kg and 200 mg/kg body weight twice daily for 21 days alongside EG administration, and a standard treatment group receiving folic acid and thiamine with EG. EG exposure resulted in significant liver damage, demonstrated by elevated serum liver enzymes (AST, ALT, ALP, GGT) and bilirubin levels, alongside histopathological liver alterations. Furthermore, antioxidant enzyme activities—superoxide dismutase, glutathione peroxidase, and catalase—were markedly reduced in the EG-treated group. ASE treatment notably ameliorated these biochemical and histological changes, suggesting its efficacy in mitigating EG-induced hepatotoxicity. The extract’s antioxidant properties and membrane-stabilizing effects likely contribute to its protective role, indicating ASE as a promising natural agent for liver protection against chemical-induced toxicity.