PHARMACEUTICAL EVALUATION BY DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF ATENOLOL IN PHARMACEUTICAL DOSAGE FORM

Objective: In this study, a simple, low cost, sensitive, highly accurate andprecise ultraviolet spectrophotometric method has been developed andvalidated for estimation of Atenolol (ATN) in tablets pharmaceutical dosageform, ATN show maximum absorbance at 274 nm in mixture of methanolwater 1:9 v/v, the methods was linear in the range 0.06-0.2 mg/ml.Methods: The method obtained with low cost term by using distilled water(DW) as major solvent 99% ATN absorbance showed linearity in theconcentration range of 0.06- 0.2 mg/ml with correlation coefficient 0.999,intercept equal to 0.0015, detection limit was found (0.00375mg/ml),quantification limit was (0.03 mg/ml), which proves suitability of proposedmethod for determination of ATL in pharmaceutical tablets dosage formaccording to the statistical analysis of the results due to high accuracy andprecession. Results: The relative standard deviations value for accuracy andprecisions were less than 0.095 with repeatability value between 103.38-100.89. Conclusions: The developed method is simple, low cost, sensitive,highly accurate and precise and easily can be used for estimation of Atenololin pharmaceutical dosage form.