Evaluation of Platelet Augmentation Potential of Setplet in Cyclophosphamide-Induced Thrombocytopenia in Wistar Rats

Setplet syrup, which contains Carica papaya, elemental iron, folic acid, cobalt, copper, vitamin B12, and vitamin C, is a unique platelet booster with hematinic properties specifically designed for dogs and cats suffering from vector-borne blood parasitic diseases. This trial aimed to evaluate the efficacy of Setplet and to optimize its supplementation level for managing thrombocytopenia. A total of 50 SD rats were divided into five groups, each consisting of 10 rats. Thrombocytopenia was induced in all groups, except for Group I (normal control), using Cyclophosphamide at a dosage of 125 mg/kg body weight for three consecutive days. Test groups II to V were then administered Setplet orally for 21 days. Blood samples were collected from the retro-orbital plexus of the rats on days 1, 7, and 21 of the study to determine platelet counts in all groups. Clotting and bleeding times were measured on the final day of the study. The trial results indicated that Setplet significantly improved hemoglobin and platelet levels while also decreasing clotting time in the rats. Our study suggests that this formulation has potential in augmenting platelet counts in various thrombocytopenic disorders and may help alleviate the Hemorrhagic complications associated with viral fever. Further support for our findings came from the assessment of bleeding time and clotting time. Both parameters remained nearly unchanged in the normal control group, but they were significantly prolonged (p<0.0001) in the disease control group, indicating the effect of decreased platelet count. In comparison to the disease control group, all treatment groups exhibited significantly reduced bleeding time (p<0.0001) and clotting time (p<0.0001).