Comparative Study of Two Spectrophotometric Methods for Simultaneous Determination of Metformin Hydrochloride and Saxagliptin

The aim of the present study was to develop, validate and compare two simple, precise and accurate UV spectrophotometric methods i.e. simultaneous equation and dual wavelength estimation method for simultaneous estimation of saxagliptin and metformin HCl in fixed dose combined oral dosage form. Both the methods satisfied the Beer-Lambert’s law in the concentration range of 2 to10 µg mL-1 for metformin and 10 to 30 µg mL-1 for saxagliptin with a low correlation coefficient. Percentage recovery of both the drugs was found to be within the limits which proved that both the methods are accurate. % RSD of precision test for both the methods was also found to be less than 2 which also suggests that the methods are precise. The developed methods were applied for estimating the drugs in marketed oral dosage form and the percentage content of both the drugs measured by the proposed methods was found to be between 98 to 101 %. To statistically compare these methods, one-way ANOVA was used and both the developed methods were found to be slightly different from each other. Hence the proposed developed methods are economical and simple and can be used for the simultaneous determination of metformin and saxagliptin in combined single dosage form.