[WIP] Reverse Vaccinology of Streptococcus iniae strain SIKU01: Appying Pangenomics and QbD to Vaccine Development

🧬 Whole Genome Assembly of 5 Streptococcus iniae bacteria isolated from diseased Asian Seabass, Thailand, comparative genomics, validation of WGS and in-sillico identification of antigens for vaccine biomanufacturing via Quality by Design (QbD). Organism: Streptococcus iniae isolates from diseased farmed Asian seabass (Lates calcarifer) Technologies: Illumina PE (short-reads) Methodologies: Single reference mapping De novo assembly Reference-guided de novo assembly Multi-reference mapping onto pangenome graphs Literature review and functional annotation of S. iniae proteome Identification of protein subset of candidate antigens based on functional annotations Pre-filtering using a QbD approach with a scoring matrix based on physico-chemical properties of Ags Second-filtering using a QbD approach with a scoring matrix based on E. coli expression system Identification of shared epitopes versus IEDB database of B- Cell epitopes in other animals Scoring and final selection of sets of best-scoring antigens for vaccine biomanufacturing using a range of downstream separation methods. NCBI Submission: Bioproject PRJNA933632 GenBank Sequence of SIKU01 Streptococcus iniae Genome