Assessment of wound healing activity of polyherbal gel, by "Excision wound Model" in Wistar rats

Objective: This study was done for comparative evaluation of polyherbal gel formulations for topical application of healing agents at the time of wound to rapidly skin repair shortly duration possible, with minor pain, malaise and scarring to the patient over the wound healing stage. Methods: Topically utilization of standard marketed ointment (Betadine) and polyherbal gel formulation prepared from the hydroalcoholic extracts (Ethanol/Methanol and water 1:1 ratio) from Azadirachta indica leaves, Calendula officinalis flower, Turmeric rhizome and Aloe-vera pulp, were prepared and assess for its effectiveness and safety. General formulation approaches towards dermal delivery feature for a wound healing medication. In the present study excision wound model was applied on wistar rats. Result: All the selected polyherbal gel formulations exhibited good stability during the storage. The skin irritation result indicates the topical application of formulated gel preparations was safe, there was no sign of any dermal reaction such as edema and erythema. Differences in wound healing were detected between various formulated polyherbal gel preparations (P<0.0001). compared to the standard marketed preparation. Which had promoting effect on the wound healing mechanism. Conclusion: The result and data obtained from the study were encouraging and hydroalcoholic extracts (ethanol and water) formulated polyherbal gel preparation (F-6 and F-9) produced better wound healing than methanol and water extracts gel preparation (F-2) and negative controlled treated animals’ groups. The result demonstrates that the formulated polyherbal gel preparations has accelerated the wound healing process and efficient in skin injuries.