Code related to the research article 'Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth'

In this repository, you can find the raw data used to generate figures for maunscript: "Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth". https://doi.org/10.1101/2022.03.01.481833 Anna Maekiniemi, Philipp Savakis, Kelly van Rossum, Jacky L. Snoep, Markus Seiler, David D. van Niekerk, Kathi Zarnack, Robert H. Singer, Evelina Tutucci Abstract Clb2 is a conserved B-type cyclin essential for mitotic progression in Saccharomyces cerevisiae, with expression tightly regulated at transcriptional and proteolytic levels. However, it remains unclear whether Clb2 protein synthesis is regulated and responsive to cell growth. Here, we show that CLB2 mRNA localizes to the bud via the She2/She3 complex, while Clb2 protein accumulates in the mother nucleus. This mRNA localization enhances translation without affecting protein localization. A structured RNA element, a ZIP-code, is located within the coding sequence and is required, but not sufficient, for both mRNA transport and protein expression. Mutation of this ZIP code disrupts mRNA localization, reduces Clb2 synthesis, increases budded phase duration and daughter cell size. In wild-type cells, Clb2 protein levels scale with bud growth, a coupling lost in ZIP code mutants. These findings reveal a mechanism by which mRNA localization and translation are coordinated to link cell growth with cell cycle progression.