Histopathological and molecular characterization of Egyptian's non melanoma skin cancer

<p><span>In humans, the most prevalent malignant neoplasms are non-melanoma skin cancers (NMSC). Basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) account for approximately 95% of all NMSC skin malignancies. In Egypt, sun exposure is responsible for more than 60% of NMSC, while skin color accounts for about 45%. The present study aimed to find sharp and accurate molecular technology to diagnose NMSC beside histopathology. 20 patients of NMSC patients were randomly selected. Tissues skin biopsies were collected from tumor and free areas for histopathological investigation and molecular analysis by random amplified polymorphic DNA (RAPD). Histopathological results revealed that 55.0% of NMSC patients had SCC, 45.0% had BCC. 35.0% of the patients were in early stage and 65.0% were in late stage, but 15.0% were in low grade and 75.0% were in high grade. 90.0% of the cases weren’t metastasized by the tumor while 10.0% were invaded by the tumor. RAPD analysis by OPA-02 primer found a significant (P=0.007) tumor marker band (2000 bp) for SCC patients and no significant bands for BCC. The study succeeded in introducing RAPD for detection of skin cancer beside histopathology.</span></p>