Antibacterial Activity of Pomegranate Peel Extract Against Listeria Monocytogenes Isolated From Refrigerated Meat

The food samples such as ice cream, Raw meat, and Refrigerated meat were randomly collected from theshops in Chennai and were analyzed for the presence of Listeria species. Totally 15 food samples wereanalyzed. The samples were introduced in Primary enrichment medium Fraser broth and incubated at30℃ for 24 hours. Then, 0.1 ml of the culture was inoculated into 10 ml of Fraser broth (Secondaryenrichment) at 37℃ for 48 hours and then plated on selective media- Oxford and PALCAM agar. Thecolonies from the selective media are streaked on TSYEA (Tryptone Soya Yeast Extract Agar). Thepresence of bacterial colonies on TSYEA (Tryptone Soya Yeast Extract Agar) confirms the presence ofListeria species. Out of 15 samples, Listeria species were detected in 9 samples. Listeria monocytogeneswas isolated, identified, and characterized from Refrigerated meat samples by Biochemical test,Haemolysis test, Carbohydrate utilization test, and CAMP test. The antibacterial activity of the Ethanolicextract of Pomegranate peel was tested against Listeria monocytogenes. 500 mg/ ml of the stock wasprepared and the zone of inhibition was measured with a diameter of 16 mm, 18 mm, and 20 mm for 25,50 and 100 .