New Analytical Method Development and Validation of Streptomycin (Anti-Biotic) Drug by RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

A new Reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the determination of in bulk and pharmaceutical dosage form. Chromatographic separation of Streptomycin was achieved by using Agilent Eclipse plus C18 (250mm ×4.6, 5µm) column. Detection was carried out at 224nm with a flow rate of 1ml/min with an injection of 20 µl was selected using mobile phase Acetonitrile and Monopotassium phosphate buffer (45:55).The standard curve was linear over a working range of 0.5-08 µg/ml and gave an average correlation factor 0.9987 for Streptomycin. The system was operated at ambient temperature and the retention time was observed at 1.870 min for Streptomycin. The method was validated with different parameters such as Linearity, Precision, Accuracy, Robustness, Limit of detection (LOD), Limit of quantification (LOQ).The Limit of detection and Limit of quantification was found to be 0.083 µg/ml and 0.28 µg/ml of Streptomycin. The relative standard deviations of intra and inter day assay less than 2 and the method was conveniently used for routine analysis of Streptomycin in bulk and tablet dosage forms.