Q-ABSORBANCE RATIO SPECTROPHOTOMETRIC METHOD OF DAPAGLIFLOZIN AND VILDAGLIPTIN IN BULK AND MARKEDED DOSAGE FORM

<p>Using a UV-visible spectrophotometer, a straight forward, accurate, Q absorbance ratio method has been developed for the instantaneous estimate of Dapagliflozin and Vildagliptin in bulk and combination tablet dose form. The ICHQ2 (R1) recommendations have been followed for all validation parameters. Using a UV-visible spectrophotometer and Methanol: Water in a ratio of (15:85) as the solvent for the preparation of the stock solution (1000 μg/ml), distilled water was used for subsequent dilutions in order to prepare working solutions. The λmax values of Dapagliflozin and Vildagliptin were found to be 195 and 220 nm, respectively. For both the drugs, in the concentration range of 5-30 µg/ml this technique followed Beer’s Lamberts law, with a 0.999 correlation coefficient for each. For Dapagliflozin, the limit of detection (LOD) was 0.649 µg/ml, or 1.962 µg/ml, and for Vildagliptin, the limit of quantification (LOQ), or 1.037 µg/ml, or 3.144 µg/ml. The drug's calculated percentage and the commercial dosage form called Daparyl-V 10/100 agreed well. Three distinct degrees of recovery investigations were carried out, and the results were deemed satisfactory. Furthermore, the outcomes of the techniques designed for precision, robustness and ruggedness are within limits. Conclusions: The recommended approach is appropriate for regular analysis of Dapagliflozin and Vildagliptin in commercial dosage forms and is inexpensive and simple to use.</p>