REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF BACLOFEN AND TRAMADOL IN BULK AND PHARMACEUTICAL DOSAGE FORM

The present research work describes a novel, simple, accurate, sensitive, rapid reversed-phase liquid chromatographic method for simultaneous estimation of Baclofen and Tramadol in bulk & pharmaceutical formulations. The chromatographic separation was achieved on WATERS HPLC with PDA detector and column Inertsil ODS, phosphate buffer and methanol as mobile phase at a flow rate of 1.0ml/min. The detection was carried out at 256 nm. The retention time of Baclofen and Tramadol was found to be 2.5 and 3.9. %Recoveries obtained for Baclofen and Tramadol were 99.85% & 100.5%.The %RSD below 2.0 shows the high precision of proposed method. The method was validated for precision, Recovery, Specify Detection & Quantification limits in accordance with ICH guidelines. KEYWORDS: Baclofen, Tramadol, RP-HPLC, Validation