The effect of Abelmoschus esculentus Ethanol Extract on TNF-αexpression in wound healing of diabetic: implications for physical recovery

Introduction: For the wound healing process, the role of pro-inflammatory mediators such as Tumor Necrotic factor (TNF-α) and Interleukin-1. TNF-α is a pro-inflammatory mediator pro-duced by macrophages that stimulates inflammatory cells, fibroblasts, and epithelial cells. One of the herbal plants suitable for treatment is okra fruit (A. esculentus) because it has beneficial properties that include antidiabetes, antioxidants, and antibacterial and anti-inflammatory properties. Objective: The research aims is to know the effect of A. esculentusethanol extract on tnf-α ex-pression in wound healing of diabetic Wistar rats.Methodology: Laboratory experimental research design with post-test-only control design us-ing male white Wistar rats (Rattus norvegicus) as a sample of 30. The treatment consisted of negative control (K-), positive control (K+), experiments with various doses of P1 dose of 300 mg/kgBB, P2 dose of 600 mg/kgBB, and P3 dose of 900 mg/kgBB extra ethanol okra fruit. On the 21st day, blood was taken through the retroorbital vein to examine TNF-alpha levels using the enzyme-linked immunosorbent assay (ELISA) method using a manual ELISA tool from Bi-oeureaux. Result and Discussion: The results of measuring TNF-α expression showed that TNF-α expres-sion in the positive control group had an average expression of 6.95 ± 0.33 and decreased by 174.35% against the negative control (K-), with an average expression of 19.06 ± 0.29. Conclusion: There is a statistically significant difference in Okra groups P1, P2, and P3. The ad-ministration of ethanol okra fruit can significantly reduce the expression of tumor necrosis fac-tor α (TNF-α) in the wound tissue of Wistar rats model of Diabetes Mellitus