Determination by High performance liquid chromatography and colorimetric of the alkaloids of Hyoscyamus muticus L. subsp falezlez (Coss.) Maire in three harvesting areas of the Algerian Sahara

Abstract Introduction: Hyoscyamus muticus L. subsp falezlez (Coss.) Maire is a Saharan species rich in tropane alkaloids (especially hyoscyamine). Hyoscyamine is raced into atropine, whose interest in pharmacy is considerable. The objective is to dose Hyoscyamus muticus L. subsp falezlez (Coss.) Maire alkaloids from the stations of Algerian Sahara (Abadla, Adrar, and Tamanrasset), to exploit the data in the valorization of this species as a potential source of industrial production of atropine. Method: The determination of tropane alkaloids (Hyoscyamine and scopolamine) concerned the whole plant (spontaneous and cultivated) and the various organs and was carried out by colorimetric method and High-performance liquid chromatography (HPLC). Results: The colorimetric assay showed that the highest level of alkaloids was observed in the Adrar Sbaa station (2.83 %) in the leaf organ. However, the stem organ showed an average level of alkaloids in all harvesting stations (from 0.5 to 0.98 %). The HPLC assay confirmed the alkaloid and hyoscyamine richness in all study stations for spontaneous plant and cultivated species. The plant of the two stations (Tamanrasset and Adrar Sbaa) stood out with grades reaching (6.693±0.555 mg/100gDM and 4.707±0.092 mg/100gDM) respectively, and a hyoscyamine rate of (5.765± 0.23 mg/100gDM) for the Tamanrasset station. Conclusions: At the end of our study and the content of the results obtained on Hyoscyamus muticus subsp falezlez (Coss.) Maire of Algeria, it is imperative to exploit this species as an industrial source of atropine production in Algeria.