Use of Pseudomonas genus for biodegradation of various plastic substrates

Microorganisms, particularly those from the genus Pseudomonas, are well recognized for their potential in bioremediation due to their ability to degrade various pollutants, including some plastic materials [1]. As plastic waste, particularly from disposable packaging, continues to pose a significant environmental challenge, there is a growing interest in utilizing microbial degradation as an eco-friendly solution. Bioremediation, the process of using microorganisms to break down pollutants, has become a key focus in addressing plastic pollution [2]. In this study, we tested five Pseudomonas strains isolated from environments contaminated with mixed plastic waste for their ability to degrade different types of plastics. Enzyme production assays were conducted on Luria-Bertani (LB) agar supplemented with polycaprolactone diol (PCLD), tributyrin, and coconut oil. Plastic degradation tests were performed on various substrates, including polyethylene terephthalate (PET), bis(2-hydroxyethyl) terephthalate (BHET), terephthalic acid (TPA), polycaprolactone (PCL), Impranil DL, polypropylene (PP), polyhydroxybutyrate (PHB), and linear low-density polyethylene (LLDPE). Among the Pseudomonas isolates tested, all strains exhibited varying degrees of degradation activity. Notably, Pseudomonas poae showed degradation on several substrates, with particularly strong activity observed on tributyrin and Impranil SD. The strain Pseudomonas sp. (marked as 111C) demonstrated exceptional degradation of TPA, BHET, tributyrin, PCLD, and Impranil SD. This strain was characterized by good growth, clear zones of degradation, and significant enzyme production, including esterases, lipases, polyesterases, and acyltransferases. This study highlights the potential of Pseudomonas species in bioremediation, offering a promising approach to mitigating plastic pollution and improving sustainable environmental management practices.