Serial Dilution

The purpose of this soil experiment is to determine if the microbes from the soil we collected are secreting anything that would have an inhibitory effect on some of the world's most notorious antibiotic-resistant pathogens. The methods to this experiment include collecting soil samples and using serial dilutions to dilute the samples to reach a reasonable number of bacterial colonies to count. We are finding the approximate number of species present with the ability to inhibit growth of other species of bacteria around them. We then use these candidates to test directly against the ESKAPE pathogens. We were successful in finding a colony that showed a clear zone of inhibition. We then transferred this bacteria onto a streak plate to get a pure sample. We now have a candidate that is a possible antibiotic-producing microbe.