Evaluation of live attenuated canine parvovirus vaccines using real-time PCR

Canine parvovirus infections pose a significant global threat to canine health, necessitating effective vaccination strategies. This study evaluates the viral content in 20 batches of live attenuated canine parvovirus vaccines using real-time polymerase chain reaction and compares the results with the immunofluorescence test. The study involved canine parvovirus strain 39, adapted to Madin-Darby canine kidney cells, and vaccine batches sourced from various local and international suppliers. The immunofluorescence test results showed 18 batches met the permissible titer level of 3 log10 fluorescent antibody infective dose 50%/mL, while two batches (3 and 18) did not. Similarly, real-time polymerase chain reaction analysis confirmed the same 18 batches met the permissible titer level, with no significant difference between the methods as indicated by the 95% confidence interval for the difference in results (lower: 4.3949, upper: 5.3600). The findings support integrating advanced diagnostic technologies like real-time polymerase chain reaction into routine vaccine evaluation protocols, ensuring higher standards of veterinary biologics assessment; this transition aims to enhance the accuracy, efficiency, and overall quality of canine parvovirus vaccine evaluation, ultimately improving canine health protection.