Anticancer Potential of Bioactive Compounds in Premna serratifolia, Premna odorata, and Premna tomentosa: A Review of In Vitro Evidence

Raden Maya Febriyanti,1,2,* Syauqi Nawwar Rafif,3,* Nazwa Nuraizza Mikdar,3,* Billa Nidia Hikmatiana,3,* Intan Timur Maisyarah,1,2 Alfi Khatib,4 Muhaimin Muhaimin1,2 1Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, West Java, Indonesia; 2Herbal Study Center, Universitas Padjadjaran, Bandung, West Java, Indonesia; 3Pharmacist Professional Study Program, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, West Java, Indonesia; 4Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Malaysia*These authors contributed equally to this workCorrespondence: Raden Maya Febriyanti, Department of Biological Pharmacy, Faculty of Pharmacy Universitas Padjadjaran, Jl. Ir. Soekarno KM. 21, Jatinangor, West Java, 45363, Indonesia, Email [email protected] Muhaimin Muhaimin, Department of Biological Pharmacy, Faculty of Pharmacy Universitas Padjadjaran, Jl. Ir. Soekarno KM. 21, Jatinangor, West Java, 45363, Indonesia, Email [email protected]: The genus Premna (Lamiaceae), widely distributed across tropical and subtropical regions, is renowned for its ethnomedicinal applications, including cardiotonic, antimicrobial, hepatoprotective, and antitumor properties. Despite these promising uses, the current body of literature relies predominantly on in vitro evidence, with limited knowledge regarding in vivo validation, metabolism, and bioavailability. This review synthesizes findings from the past decade on the Premna serratifolia group (P. serratifolia, P. odorata, and P. tomentosa), focusing on their bioactive compounds and mechanisms of in vitro anticancer activity. The compounds identified—such as flavonoids, terpenoids, and steroids—exhibit diverse actions, including cell cycle arrest, apoptosis induction, inhibition of metastasis, oxidative stress modulation, and autophagy. Key compounds like quercetin, kaempferol, and stigmasterol demonstrate multi-targeted actions, effectively regulating pathways such as PI3K/AKT and NF-κB while selectively targeting cancer cells. These findings underscore chemopreventive potential from P. serratifolia group and their ability to complement conventional cancer therapies, potentially reducing side effects and overcoming drug resistance. Furthermore, the review validates the ethnomedicinal use of Premna species and bridges traditional knowledge with modern oncology. However, the absence of comprehensive in vivo and clinical data warrants further research to fully harness these compounds’ potential. This study highlights P. serratifolia, P. odorata, and P. tomentosa as promising sources for novel plant-derived anticancer agents, offering opportunities for future drug discovery.Keywords: phytochemicals, natural product-based therapy, chemoprevention, cytotoxic activity, multi-target mechanisms