Антиадгезивні властивості 4-(адамантил-1)-1-(1-амінобутил)бензолу щодо Staphylococcus aureus

Background. Staphylococcus aureus is classified as a bacterium with a high level of antibiotic resistance, one of the contributing factors being its ability to form biofilms. Targeting the early stages of biofilm formation is a promising strategy for antimicrobial therapy in patients with biofilm-associated infections. Objective. To determine the ability of 4-(adamantyl-1)-1-(1-aminobutyl)benzole to influence the formation of S. aureus biofilms and the expression of biofilm formation genes. Methods. The minimum inhibitory concentration (MIC) of 4-(adamantyl-1)-1-(1-aminobutyl)benzole (code AM-166) against the methicillin-resistant S. aureus strain 222 was determined using the serial microdilution method. The antibiofilm activity of AM-166 was studied using O'Toole's method, and the intensity of S. aureus biofilm formation was assessed according to Stepanovic. The effect of AM-166 on gene expression was investigated using real-time PCR. Results. The MIC of AM-166 against S. aureus 222 was determined to be 5 μg/ml. In the presence of AM-166 at 0.25 and 0.5 MIC, S. aureus biofilm formation decreased from moderate (control) to weak, while at 5.0 MIC, the strain completely lost its ability to form biofilms. It was found that at a concentration of 0.5 MIC, AM-166 increased the expression of the icaR gene while decreasing the transcriptional activity of the icaA, clfB, fib, fnbB, ebpS, and eno genes, which are involved in biofilm formation and adhesion. Conclusions. The adamantane derivative AM-166 disrupts MRSA biofilm formation, alters the transcriptional activity of ica-locus genes, and inhibits S. aureus attachment to biotic surfaces by affecting gene expression.