The Effects of Magnesium Glycinate vs. Magnesium Citrate on the Growth of Lactobacillus acidophilus in Simulated Gastrointestinal Conditions

Despite numerous studies on the importance of magnesium intake, little is known about the variations between different forms of magnesium and their interactions with gut bacteria. Magnesium is essential for many bodily functions, and its absorption in the intestines is key for maintaining levels that support muscle function, nerve transmission, and energy production; however, its effects on probiotics have not been thoroughly investigated. With many magnesium supplements available on the market, questions arise regarding which is most effective. This study aimed to evaluate and compare the efficacy of magnesium glycinate versus magnesium citrate in promoting the absorption of Lactobacillus acidophilus, and to determine which form enhances uptake more effectively. It was hypothesized that the addition of magnesium glycinate or citrate to a L. acidophilus mixture would improve absorbance, with glycinate yielding higher results. Three experimental groups were examined with the addition of L. acidophilus in the MRS broth: one group without magnesium, one with magnesium glycinate, and one with magnesium citrate. Using a SpectroVis Plus spectrophotometer, absorbance units (AU) were assessed based on the intensity of transmitted light. The one-way ANOVA test, conducted with an alpha level of 0.05, revealed significant differences between the control group and each of the experimental groups (F(2, 87) = 12.54, p < 0.001). Therefore, it was concluded that there was a significant difference in AU between the control group and the magnesium citrate and magnesium glycinate trials.