Comparative Proteomic Analysis of Endolymphatic Sac Luminal Fluid in Patients with Meniere’s Disease and Controls

Zhiwen Zhang,1,* Qin Wang,1,* Zhou Zhou,1 Anquan Peng,1 Wenqi Jiang2 1Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital, Central South University Changsha, People’s Republic of China; 2Department of Anesthesiology, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wenqi Jiang, Department of Anesthesiology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, People’s Republic of China, Tel +86-13570399853, Email [email protected]: Meniere’s disease (MD) is known to be caused by the dysfunction of the endolymphatic sac (ES), but its molecular mechanism is unknown.Methods: We performed a comparative proteomic analysis of ES luminal fluids (ELFs) from patients with MD and controls.Results: We found 6 differentially expressed proteins, including 2 significantly increased proteins and 4 significantly decreased proteins, 8 proteins identified exclusively in at least 7 of the 8 ELF samples from MD patients and 3 proteins detected solely in at least 4 of the 5 ELF samples from controls.Discussion: The increased levels of IGLV 3– 9 and IGLV1-47 in MD group compared with control group suggested an increased inflammatory reactions and a decreased level of Aldehyde dehydrogenase 2 in MD group compared with control group might result in oxidative damage and inflammatory lesions in the ES of MD. Whereas CD44 identified exclusively in MD samples might be involved in the metabolism of its ligand, hyaluronic acid for overproduction of endolymph in the ES of MD.Keywords: proteomic, meniere’s disease, endolymphatic sac, endolymphatic sac luminal fluid