Isolation and Identification of Antibiotic-Producing Bacteria from Garden Soil

This study aims to isolate and identify bacteria with potential antimicrobial properties from a garden soil sample. Using aseptic techniques, bacteria were cultured and distinguished based on colony morphology. Serial dilution and streak plate methods were employed to obtain pure isolates. Antimicrobial activity was assessed through the recognition of zones of inhibition against test organisms. Gram staining was performed to determine whether the isolates were Gram-positive or Gram-negative. The findings contribute to the ongoing exploration of soil microbiota as a source of new antibiotics and provide insights into the microbial diversity present in garden soil.