Decrease of Resolvin D1 and E1 in Patients with Chronic Epilepsy, Implicating Dysfunction of Neuroinflammation Resolution

Hanli Li,1,2 Zhong Dong,2 Yujing Yang,3 Qibing Sun,2 Fengqing Lu,2 Ran Li,2 Weifeng Zhou,1 Wei Gui,3 Rupan Gao,4 Yu Wang2 1School of Clinical Medicine, Anhui Medical College, Hefei, People’s Republic of China; 2Department of Neurology, the First Affiliated Hospital of Anhui Medical University, Hefei, People’s Republic of China; 3Department of Neurology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, People’s Republic of China; 4Department of Hematology, Zhongshan Hospital Fudan University, Shanghai, People’s Republic of ChinaCorrespondence: Rupan Gao, Department of Hematology, Zhongshan Hospital Fudan University, Shanghai, People’s Republic of China, Email [email protected] Yu Wang, Department of Neurology, The First Affiliated Hospital of Anhui Medical University, Hefei, People’s Republic of China, Email [email protected]: Inflammation resolution is mediated by specialized pro-resolving lipid mediators (SPMs). It’s of high interest to understand the alterations of SPMs in chronic epilepsy.Methods: Sixty-five patients with chronic epilepsy, 43 healthy controls and 43 patients with non-inflammatory neurological disorders were enrolled in this study. Plasma and cerebrospinal fluid (CSF) levels of SPMs were measured using liquid chromatography-tandem mass spectrometry (LC-MS-MS). Moreover, Resolvin D1 (RvD1) and resolvin E1 (RvE1), as well as pro-inflammatory factors were analyzed by enzyme-linked immunosorbent assay (ELISA).Results: LC-MS-MS found several lipid mediators were altered, especially plasma (P = 0.014) and CSF (P = 0.010) RvE1 levels were decreased in patients with chronic epilepsy. Furthermore, ELISA results showed that in plasma and CSF, RvD1 and RvE1 levels were both lower in patients with chronic epilepsy, while pro-inflammatory factors were higher than in controls. Moreover, plasma RvE1 level was independently negatively associated with the National Hospital Seizure Severity Scale scores (&Bgr; = − 0.00636, P