Mechanism Exploration within SAM-RNA for Drug Immunity

Breast cancer is one of the most rampant malignancies in women around the world. The treatment for breast cancer consists of, but is not limited to, surgery, chemotherapy, endocrine therapy, and radiotherapy. Tamoxifen and Paclitaxel are the cornerstones of endocrine therapy drugs and chemotherapy drugs, respectively. Drug resistance, which includes primary resistance and consecutive resistance, is the biggest reason for treatment failure. Treatment failure results in disease relapsing and metastasis. Therefore, it is very important to explore the mechanism of endocrine resistance and chemotherapy resistance in breast cancer. Previously we designed and constructed a RNA Library full of fragments consisting of random 19bp through molecular cloning. From there, we found that Sam RNA1 and Sam RNA7 may play important roles in drug resistance through preliminary experiments. The objectives of this project is to construct Sam RNA overexpressing drug-resistant cell lines of breast cancer, to explore the effect of Sam RNA1 and Sam RNA7 in Tamoxifen resistance and Paclitaxel resistance, and to explore the mechanism of Sam RNA1 and Sam RNA7 in drug resistance. Further, we will attempt to find predictors of drug immunity within these cell lines.