Isolation and Testing of the Antibiotic Compounds Produced by Bacteria “AB24D”

Antibiotic resistance is a growing global threat that renders many of our current treatments ineffective leading to prolonged illness and increased mortality. This work is important because it explores whether the bacterium “AB24D” might offer new strategies to combat drug-resistant infections. “AB24D” is a strain of pseudomonas. In this research, compounds produced by bacteria "AB24D” were extracted and evaluated for their effectiveness as antibiotics. Several methods, including thin-layer chromatography, column chromatography, and HPLC were used to separate the mixtures. The fractions were then tested against tester strain of bacteria in broth. The results from the separations and testing will be discussed.