The Effect of Nanoparticles and Silver Ions on the Concentration of Protein and Amino Acids in Soil Columns

Soils are continuously exposed to large amounts of engineered nanoparticles, particularly silver nanoparticles (AgNPs), which can affect soil microbial activities and nitrogen cycling. The hypotheses of the present study were: (i) vegetation types would differ in their responses to Ag types and concentrations, (ii) these responses would be linked to changes in soil protein and amino acid concentrations, and (iii) combined plant root systems alongside Ag types and concentrations would have offsetting effects on soil protein and amino acid concentrations. A greenhouse experiment was conducted to test these hypotheses using a factorial arrangement of treatments within a randomized block design. Two soil types with loamy sand and sandy loam textures were collected from agricultural fields in Isfahan, specifically from the Badroud (33◦ 44′ 50" N, 51◦ 57′ 55" E) and Femi (33◦ 42′ 17" N, 51◦ 59′58" E) regions. The treatments included: 1) soil types (loamy sand and sandy loam), 2) root systems (non-planted, wheat, and safflower), 3) Ag types (no Ag added, AgNPs, and AgNO3), and 4) Ag concentrations (50 and 100 ppm). The plants were harvested 110 days after sowing, with soil samples collected from both the root zone and non-planted soil, after which the concentrations of protein and amino acids were measured. In the Badroud soil, protein concentration significantly decreased (p < 0.05) with increasing depth. Although depth changes did not show a significant difference in protein concentration in the soil under wheat cultivation, increasing depth resulted in a significant decrease (p < 0.05) in protein concentration in the soil under safflower cultivation. In the Fami soil, the addition of silver nitrate led to a significant (p < 0.05) increase in protein concentration, despite the fact that the addition of silver nanoparticles had no significant (p < 0.05) effect on soil protein concentration. In the Badroud soil, the highest concentration of soil amino acids was observed in the silver nitrate treatment, while the silver nanoparticle treatment did not significantly affect soil amino acid concentrations (p < 0.05). However, applying silver treatments at both tested concentrations resulted in a significant increase (p < 0.05) in soil amino acid levels. Overall, the effects of nanoparticles varied depending on the measured parameters (protein or amino acid), soil texture, and type of cultivation. Further studies are needed to determine the mechanisms by which AgNPs and AgNO3 affect the soil nitrogen cycle in the presence of plants at different soil depths.