Chromatographic Separation and Testing of Compounds Produced by a Pseudomonas Bacteria

“Goldilocks” is the working name of a bacteria collected by a JCCC microbiology student. It has previously been shown to inhibit S. epi when grown competitively. Chemical extracts taken from these bacteria inhibit S. epi when grown in broth with the extract. This research explored the separation and isolation of components of the extract mixture using chromatography. The separated fractions were tested for antimicrobial activity in broth. The results will be discussed.